Download A Laboratory Guide to Glycoconjugate Analysis by Uwe D. Neue, Charles H. Phoebe (auth.), Dr. Peter Jackson, PDF

By Uwe D. Neue, Charles H. Phoebe (auth.), Dr. Peter Jackson, Prof. John T. Gallagher (eds.)

18. 2 precept of FACE/Gel Retardation Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 349 18. three Labelling of Oligosaccharides with ANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 350 18. four Screening of Carbohydrate Ligands for Proteins . . . . . . . . . . . . . . . . . . . . . . . . . . . 352 18. five size of Binding consistent for the interplay among Protein and ANTS-Labelled Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 355 18. 6 dimension of Binding consistent for the interplay among Protein and local Carbohydrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 357 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 360 ~ the applying of Capillary Affinity Electrophoresis to the research _ of Carbohydrate-Protein Interactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 1 creation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 361 19. 2 precept of CAE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 363 19. three choice of organization Constants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 364 19. four Technical approaches . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 basic concerns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 366 19. five boundaries of the procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 370 19. 6 program of CAE to the research of Carbohydrate-Protein Interactions . . . . . . 371 19. 7 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 377 20. 1 advent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 379 Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 380 20. 2 Technical methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 381 20. three pattern Detection and pattern restoration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Autoradiography and marking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 pattern detection by means of blotting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 389 Semipreparative ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 390 20. four research of information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 391 Measuring pattern mobilities - calculating a retardation coefficient . . . . . . . . . . . . 391 Graphical research of knowledge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 392 reading ACE styles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 393 opposite ACE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 395 20. five precis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 397 Acknowledgements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 398 topic Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 399 XII record of participants Nebojsa Avdalovic John T. Gallagher Dionex company melanoma learn crusade division of clinical Oncology 445 Lakeside force college of Manchester Sunnyvale, CA 94086 Christie CRC examine Centre Klaus Biemann Wilmslow highway division of Chemistry Manchester M20 4BX Massachusetts Institute of expertise united kingdom Cambridge, MA 02139-4307 united states Geoffrey R.

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Larger oligosaccharides are retained longer than are smaller oligosaccharides, and for equal molecular weight the ones with a larger hydrophilic area are retained more (39). Gradient elution from high acetonitrile to high water content is preferentially used to elute structures of widely different compositions. This technique is also compatible with detection at low ultraviolet (UV) light. As mentioned in the previous paragraph, HILIC is used as a second step after the preliminary separation of the glycans by charge.

In: G. ) Comprehensive Biochemistry, Vol. 19, part 2, p. I, Elsevier, Amsterdam. 9 Haltiwagner RS, Kelly WG, Blomberg MA, Dong D, Roquermone E and Hart GW (1992) Biochem. Soc. Trans. 20, 264. 2 Varki A (1993) Glycobiology 3: 97. \0 Laemmli UK (1970) Nature 277: 680. D. Hannes and D. 4 of the sugar chains of glycoproteins. Eur. Rickwood (eds) An Introduction to Polya- 1. Biochem. 209: 483. cylamide Gel Electrophoresis: A Practical Dwek RA, Edge Cl, Harvey 01, Wormald Approach, IRL, Oxford. MR and Parekh RB (1993) Glycoform analysis of glycoproteins.

Chromatogr. 126: 731 22 D ' Amboise M, Noel D and Hanai T (1980) Carbohydr. Res. 79: I 23 Mopper K and Degens ET (1972) Anal. Biochem. 45: 147 24 Orth P and Engelhardt H (1982) Chromatographia 15: 91 25 Verhaar LATh and Kuster BFM (1982) J. Chromatogr. 234: 57 20 High-Performance Uquid Chrometogrephy of Derivetized end Non-Derivetized OIigoseccherides: ARevi_ 26 Samuelson 0 and Havlicek 1 (1975) Anal. Chern. 47: 1854 27 Neue UD (1973) Master's Degree Thesis, Universitat des Saari andes 28 Koizumi K, Utamura T, Kubota Y and Hizukuri S (1987) 1.

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